Description
Description: Full-length recombinant human PDE1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE1B is a member of the PDE protein superfamily that catalyzes the hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1B is a calmodulin-dependent enzyme that is stimulated by calcium-calmodulin complex (1). PDE1B is express in the lymphoblastic and leukemic cell lines and causes the cells to undergo apoptosis, suggesting PDE1B could be a novel therapeutic strategy for the treatment of leukemia (2). GM-CSF is a major regulator of monocyte to macrophage differentiation and this cytokine has been shown to up-regulate PDE1B